Development of an At-Column Dilution Modulator for Flexible and Precise Control of Dilution Factors to Overcome Mobile Phase Incompatibility in Comprehensive Two-Dimensional Liquid Chromatography.

Abstract

With the combination of different mechanisms, two-dimensional liquid chromatography has brought revolutionary changes compared to the traditional one-dimensional separation, which dramatically improve the peak capacity in separation and meet the ever-increasing demand for the analysis of complex samples in different research fields, such as chemistry, medicine, etc. However, the incompatibilities between two columns due to the transport of the large sample volume and the solvent effect always limit the wide use of two-dimensional liquid chromatography. In order to resolve this problem an at-column dilution (ACD) modulator was established to overcome the solvent incompatibility in the orthogonal combination within the comprehensive two-dimensional liquid chromatography. This interface is modified from normal two-dimensional interfaces by an additional transfer pump, which realize the at-column dilution without a flow split during the transportation. Moreover, with the control of the transfer flow and the second-dimensional gradient flow, it is able to precisely regulate the at-column dilution factor and conveniently optimize the separation conditions in both dimensions. In this work, a systematic research has been done between the setups with/without the at-column interface in the combination of reversed-phase liquid chromatography and hydrophilic interaction chromatography (RPLC × HILIC) and HILIC × RPLC, which proved that the at-column interface is able to resolve the solvent conflict problem very well. Furthermore, red ginseng was chosen as a real sample to investigate the applicability of the at-column dilution modulator for comprehensive two-dimensional chromatography with high orthogonality.