Abstract
Developing the rapid, simple and sensitive biosensor system for tetracycline detection is very important in food safety. In this paper, we developed a label-free aptasensor for electrochemical detection of tetracycline. The reorganization of tetracycline binding aptamer was confirmed by Isothermal Titration Calorimetry, Kd = 5.18 × 10−5 mol L−1. According to the electrochemical impendence spectroscopy (EIS) analysis, there was a linear relationship between the log concentration of tetracycline and the charge transfer resistance (ΔRet) from 5.0 to 5.0 × 103 ng mL−1 of the tetracycline conc. The detection limit was 1.0 ng mL−1 within a detection time of 15 min. The average of assemble rate Q was at 82.4% with a differential batches' RSD of 4.6%. The current change of this aptasensor lies within at 8.5% after a storage of 15 days under 4 °C. The result aptasensor had shown a good reproducibility with an acceptable stability in tetracycline detection. The recoveries of TET in spiked milk samples were in the range of 90.0–95.7%.
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