Development of an Analytical Method for the Determination of Metals in Chicken Breast by Microwave Induced Plasma Optical Emission Spectrometry (MIP-OES)

Ane Martiele Pinto,Anderson Ribeiro,Mariana Vieira,Ana Carla Boeira,Meibel Lisboa,Aline Medina

doi:10.21577/0103-5053.20190151

Abstract

An analytical method for the determination of Al, Ca, Cd, Cr, Cu, Fe, Hg, K, Mg, Na and Zn in breast of conventional, country and Christmas chicken and turkey samples using microwave induced plasma optical emission spectrometry (MIP-OES) technique was developed. Samples were decomposed in a digester block with a reflux system under conditions optimized using a central composite design as it follows: 2 g of sample, in 7.5 mL of HNO3 during 180 min at 160 °C. The accuracy was evaluated by the analysis of standard reference material SRM 1546 meat homogenate (94 to 108%) and also by addition and recovery tests (80 to 121%). The highest concentrations, in mg kg-1, of Al (2.77), Ca (88.3), Cu (1.99), Fe (3.65), K (3236), Mg (292.62) and Na (312.03) were found in the breast of conventional chicken sample. Zinc presented high value in the breast of turkey sample (10.6 mg kg-1). However, the found concentrations were lower than the daily consumption limit established by supervisory agencies and reference limits from Brazilian legislation. Cadmium, Cr and Hg presented values below limit of quantification. In this way, it is evident that chicken breast should be consumed in a complementary way in a healthy diet.

Highlights

According to the Brazilian Animal Protein Association (ABPA), few countries in the world have the propensity that Brazil naturally achieved to be recognized internationally as the “Barn of the World” due to extensive grain fields with fertile land and an exceptionally favorable climate
Considering the importance of chicken meat food security, the present study aims to develop an analytical method for the quantification of Al, Ca, Cd, Cr, Cu, Fe, Hg, K, Mg, Na and Zn in chicken breast samples by MIP‐OES
Figures of merit for the determination of the analytes Al, Ca, Cd, Cr, Cu, Fe, Hg, K, Mg, Na and Zn in chicken breast samples by microwave induced plasma optical emission spectrometry (MIP-OES) were obtained from calibration curves data and the two sample introduction systems were used: conventional nebulization and multimode sample introduction system (MSIS) (Tables S5 and S6, respectively, SI section)

Summary

Introduction

According to the Brazilian Animal Protein Association (ABPA), few countries in the world have the propensity that Brazil naturally achieved to be recognized internationally as the “Barn of the World” due to extensive grain fields with fertile land and an exceptionally favorable climate. Reflux systems for acid decomposition have recently been applied, providing satisfactory results for the determination of metal and volatile elements in different samples.[21,22,23,24] The cold finger (reflux system) provide a larger surface area for the condensation and allow the formation of the absorber solution film for better retention of the volatile species In this way, losses of analyte by volatilization and reposition of acids are avoided, increasing the efficiency of decomposition.[21]. Considering the importance of chicken meat food security, the present study aims to develop an analytical method for the quantification of Al, Ca, Cd, Cr, Cu, Fe, Hg, K, Mg, Na and Zn in chicken breast samples (conventional chicken, country and Christmas chicken and turkey) by MIP‐OES.

Optimization procedures

Results and Discussion

Conclusions