Development of an Analytical Method for Simultaneous Determination of the Modified Forms of 4,15-Diacetoxyscirpenol and their Occurrence in Japanese Retail Food.

Tomoya Yoshinari,Maiko Watanabe,Nanami Takeda,Yoshiko Sugita-Konishi

doi:10.3390/toxins10050178

Tomoya Yoshinari, Maiko Watanabe + Show 2 more

Open Access

https://doi.org/10.3390/toxins10050178

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Journal: Toxins	Publication Date: Apr 26, 2018
Citations: 13	License type: CC BY 4.0

Affiliation: National Institute of Health Sciences, Azabu University

Abstract

4,15-Diacetoxyscirpenol (4,15-DAS) is a type A trichothecene mycotoxin produced by Fusarium species. Four modified forms of 4,15-DAS including 7-hydroxydiacetoxyscirpenol, 7,8-dihydroxydiacetoxyscirpenol, 4β,8α,15-triacetoxy-3α,7α-dihydroxy-12,13-epoxytrichothec-9-ene and 4,15-diacetylnivalenol were purified from cultures of F. equiseti. An analytical method using a multifunctional column has been developed for the simultaneous determination of 4,15-DAS, its four modified forms, T-2 toxin, HT-2 toxin and neosolaniol in cereals. The performance of the current method was evaluated, and a total of 248 samples of five different commodities were analyzed for over two years by this method. 4,15-DAS was detected in Job’s tears products, corn flour and azuki bean, but it was not found in wheat flour or rye flour. The four modified forms of 4,15-DAS were detected in samples of Job’s tears products, contaminated by 4,15-DAS. This is the first report on quantification of the modified forms of 4,15-DAS in cereals.

Highlights

Trichothecene mycotoxins are a group of more than 80 compounds mainly produced by the Fusarium species
These trichothecene mycotoxins were detected in a variety of foods including wheat, barley, Job’s tears products, beer, corn flour and azuki bean. 4,15-Diacetoxyscirpenol
(4,15-DAS, Figure 1) is a type A trichothecene mycotoxin produced by some Fusarium species including F. equiseti, F. poae and F. sporotrichioides [3,4]

Summary

Introduction

Trichothecene mycotoxins are a group of more than 80 compounds mainly produced by the Fusarium species They are classified into four types (A-D) based on their structures [1]. Our group performed a survey of the contamination of cereals and processed cereal-based foods by deoxynivalenol (DON), a type B trichothecene, and two type A trichothecene mycotoxins, T-2 toxin and HT-2 toxin, between 2010 and 2012 [2]. These trichothecene mycotoxins were detected in a variety of foods including wheat, barley, Job’s tears products, beer, corn flour and azuki bean. As with other trichothecene mycotoxins, acute oral exposure of 4,15-DAS causes diarrhea, lethargy and vomiting in animal models [5,6]

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