Abstract
The study examined the effect of human Chorionic Gonadotrophin (hCG) and/or Insulin Transferring Sodium Selenite (ITS) on the proliferation and development of rat Leydig cells purified by Nycodenz gradient. Leydig cells purity, viability and proliferation after purification and 3 days of cultured were evaluated. Leydig cells 1 × 106 cells/ml were cultured in DMEM containing 10% Newborn Calf Serum (NBCS) and divided into four kinds of treatments 1) as a control, 2) control + 2.5 IU/ml hCG, 3) control + ITS (5 μg/ml insulin, 10 μg/ml transferrin, 5 μg/ml Se), and 4) control + hCG + ITS. Leydig cells purification results showed 91.40% of purity, viability was 98.17% and concentration 7.03 × 106 cells/ml. The addition of ITS and hCG + ITS in DMEM produced Leydig cell proliferation by 88.35% and 90.64% higher than in controls (86.82%) (p p p
Highlights
Androgen hormonal therapy for hypogonadism in men has been done to maintain testosterone level physiologically
The results showed that a percentage of Leydig cells after purified with Nycodenz gradients were 91.40%, while the viability was 98.17% and the cell concentration was 6.30 × 106 cells/ml (Table 1)
Leydig cells proliferation were cultured in DMEM + Insulin Transfferin Sodium Selenite (ITS) and DMEM + human Choriogenic Gonadotrophin (hCG) + ITS significantly (p < 0.05) higher at 88.35% and 90.64% when compared to control (86.82%)
Summary
Androgen hormonal therapy for hypogonadism in men has been done to maintain testosterone level physiologically. Bhasin & Bremer stated that this therapy can increase muscle strength, improve osteoporosis, stabilized bone density and restore the secondary sexual characters [1] Provision of these hormones in the long term can cause an increase in blood viscosity, red blood cell formation abnormalities, hypertension, stroke, bone density changes and emotional changes [1,2,3]. It is necessary to attempt an alternative therapy such as Leydig cells transplantation which naturally produced testosterone that can be used to replace the use of synthetic testosterone hormone [1]. This cellular therapy has limitations in terms of availability of tissue and cells.
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