Development of Amino Acids Functionalized SBA-15 for the Improvement of Protein Adsorption.

Raquel Gutiérrez-Climente,Jérémie Gouyon,Pascal Dumy,Catherine Perrin,Pascal Etienne,Margaux Clavié,Gilles Subra,Pierre Martineau,Ahmad Mehdi,Martine Pugnière,Giang Ngo,Yoann Ladner

doi:10.3390/molecules26196085

Abstract

Ordered mesoporous materials and their modification with multiple functional groups are of wide scientific interest for many applications involving interaction with biological systems and biomolecules (e.g., catalysis, separation, sensor design, nano-science or drug delivery). In particular, the immobilization of enzymes onto solid supports is highly attractive for industry and synthetic chemistry, as it allows the development of stable and cheap biocatalysts. In this context, we developed novel silylated amino acid derivatives (Si-AA-NH2) that have been immobilized onto SBA-15 materials in biocompatible conditions avoiding the use of toxic catalyst, solvents or reagents. The resulting amino acid-functionalized materials (SBA-15@AA) were characterized by XRD, TGA, EA, Zeta potential, nitrogen sorption and FT-IR. Differences of the physical properties (e.g., charges) were observed while the structural ones remained unchanged. The adsorption of the enzyme lysozyme (Lyz) onto the resulting functionalized SBA-15@AA materials was evaluated at different pHs. The presence of different functional groups compared with bare SBA-15 showed better adsorption results, for example, 79.6 nmol of Lyz adsorbed per m2 of SBA-15@Tyr compared with the 44.9 nmol/m2 of the bare SBA-15.

Highlights

Ordered mesoporous siliceous materials have been intensively investigated as supports for enzyme or protein immobilization and have found applications in bioseparation, enzyme catalysis, biosensors, and drug delivery [1,2,3,4,5]
The hydrothermal treatment required for its preparation is carried out at high temperatures (e.g., 130 ◦ C) [9], reducing the microporosity in favor of macroporosity, and stabilizing the structure of the SBA-15 compared to others
We prepared a set of amino acids (AA) with different types of sides chains, in order to mimic the diversity of protein surfaces, in the context of a protein sequence where both the N and

Summary

Introduction

Ordered mesoporous siliceous materials have been intensively investigated as supports for enzyme or protein immobilization and have found applications in bioseparation, enzyme catalysis, biosensors, and drug delivery [1,2,3,4,5]. Barbara Amorphous material (SBA-15) [6,7] is the most widely used. Compared to other hexagonal mesostructured materials such as the MCM-41 [7], it presents a wider pore size distribution (5–30 nm) and thicker walls. Those properties give SBA-15 a high surface area and an improved thermal, mechanical and chemical resistance [8]. The hydrothermal treatment required for its preparation is carried out at high temperatures (e.g., 130 ◦ C) [9], reducing the microporosity in favor of macroporosity, and stabilizing the structure of the SBA-15 compared to others

Objectives

Methods

Results

Conclusion