Abstract
Sugarcane is considered as an industrial crop that produces sugar. The number of transgenic sugarcane on the market is currently increasing. Therefore, investigation of the potential allergens and toxics in transgenic sugarcane is necessary, since there is less information regarding food safety for human consumption. Bioinformatics and experimental analysis were used for the validation of the allergenic potential of transgenic sugarcane overexpressing sucrose–phosphate synthase (SPS). Bioinformatics analysis showed that SPS has no homology with any known allergenic proteins. However, eight-residues identical contiguous sequence was detected, and further specific assessment is required to confirm the potential of allergenicity. The results of protein stability evaluation showed that SPS gradually decreased at 28 °C and rapidly inactivated at 60 °C and 90 °C by heat treatment. In addition, total protein was degraded by simulated gastric fluids (SGF), and simulated intestine fluid (SIF) assays for one-minute incubation. The level of specific IgE in the transgenic sugarcane and controls also showed no potential risk of allergy. An acute oral toxicity assay was performed by oral gavage of transgenic sugarcane juice in mice. The LD50 for transgenic sugarcane juice was >25 gr/kg body weight. We propose a development method for allergenicity and toxicity assessment in transgenic sugarcane.
Highlights
In agricultural biotechnology, genetically engineered products have triggered a range of social and ethical concerns
Agronomy 2019, 9, 23 the guidelines of the World Health Organizationn (WHO) and Food and Agriculture Organization (FAO) [1] in the Codex Alimentarius document, several risk assessments are essential to determine whether transgenic crops are safe or not
The first step of allergenicity testing on transgenic crops is to identify potential allergens using bioinformatics methods
Summary
Genetically engineered products have triggered a range of social and ethical concerns. Agronomy 2019, 9, 23 the guidelines of the World Health Organizationn (WHO) and Food and Agriculture Organization (FAO) [1] in the Codex Alimentarius document, several risk assessments are essential to determine whether transgenic crops are safe or not. There are several reports evaluating transgenic crops, including allergenicity and toxicity analysis [2,3,4] since the substitution or insertion of genetic material has raised risks the issue in allergenicity. The first step of allergenicity testing on transgenic crops is to identify potential allergens using bioinformatics methods. A toxicity study, in parallel with an allergenicity assessment, has been used to assess potential hazards to human through the acute exposure of genetically engineered products to animal model testing [4]
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