Abstract

Indonesia has a lot of high potential seaweed as a source of an alginate, but the extraction method had been used was not suitable. The aim of this research is to develop alginate extraction method from Turbinaria ornata seaweed with calcium method and compare the cost of extraction with an acid method as a control. In this research, various concentration of calcium chloride (CaCl2) was used in the separation of alginate from the extracted filtrate. The concentration of CaCl2 used varies from 0.50; 0.75; 1.00; 1.25; and 1.50 M. For comparison, alginate extraction with acid (control) method was used. Alginate quality parameters observed included alginate yield, product color degree, and gel viscosity. The results showed that the yield of alginate produced by calcium method was 36.89; 44.00; 56.00; 52.89 and 53.11% and for control 22.45%. From the degree of product color, CaCl2 concentration did not significantly affect the color of alginate but was darker when compared to the product extracted by the acid method. The viscosity of alginate produced by calcium method was 27,69; 26,57; 24.50; 22.41 and 19.92 cP while for control 32.88 cP. The extraction of Na-alginate with calcium method can decrease the need for Na-alginate extraction cost by 85% from the amount of Na-alginate extraction cost requirement by the acid method.

Highlights

  • Alginat adalah kelompok polisakarida anionik alami berasal dari dinding sel rumput laut (Szekalska et al 2016), misalnya

  • The results showed that the yield of alginate produced by calcium method was 36.89; 44.00; 56.00; 52.89 and 53.11% and for control 22.45%

  • Jakarta: Pusat Riset Pengolahan Produk danSosial Ekonomi Kelautan dan Perikanan, Badan Riset Kelautan danPerikanan, Departemen Kelautan dan Perikanan

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Summary

BAHAN DAN METODE Bahan dan Alat

Bahan baku rumput laut cokelat yang digunakan adalah T. ornata yang diperoleh dari Pantai Sepanjang, Gunung Kidul, Provinsi D.I Yogyakarta. Bahan kimia yang digunakan untuk proses ekstraksi alginat dengan metode kalsium adalah: CH2O, HCl (Mallinckrodt Chemicals, USA), Na2CO3, CaCl2 (KGaA, Germany), C2H5OH, isopropil alkohol (IPA) (J.T.Baker). Alat yang digunakan adalah waterbath (Shibata, Tokyo), pengaduk/spatula, gelas beker, gelas ukur (Herma), kain saring, saringan plastik (Test Sieve I.S.O, Tokyo), alat penggiling, timbangan digital (Shimadzu 8X320D), alat pengepres, termometer, pH meter (Hana), viscometer (Brookfield) dan chromameter (CR300 Konika Minolta)

Metode Penelitian Ekstraksi alginat
HASIL DAN PEMBAHASAN Rendemen
Derajat Warna
Findings
Metode Kalsium dan Metode Asam
Full Text
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