Abstract
In this paper, using molybdenum disulfide doped with gold nanoclusters (Au NCs@MoS2) as the substrate, an aflatoxin aptamer sensor was constructed utilizing iron porphyrin organic porous material modified with streptavidin and loaded with gold nano-bipyramids (SA-Au NBPs@ porous aromatic framework (PAF)-40-Fe) as a label. The catalyzed reduction current of hydrogen peroxide (H2O2) by Au NBPs@PAF-40-Fe was detected which is proportional to the concentration of aflatoxin B1. Thereby, quantitative detection of aflatoxin B1 (AFB1) is achieved. Under the optimal conditions, the linear range of the sensor is 0.05 ~ 85 ng/mL with detection limit of 0.017 ng/mL and the linear correlation coefficient (R2) of 0.9825. The average recovery is 99.28 %. This aflatoxin aptamer sensor has good selectivity and provides a highly sensitive method for the detection of aflatoxin.
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