Abstract
A protocol was optimized for genetic transformation of Rohida ( Tecomella undulata) from cotyledonary node tissues using Agrobacterium tumefaciens strain GV2260 harboring binary vector pBinAR containing osmotin and nptII gene under control of CaMV35S promoter. This is the first report on the transformation of T. undulata. The effective concentration of selectable marker antibiotic used for screening of transformants was 85.97 μM in shooting media and 42.98 μM in rooting media. PCR and Southern blot confirmed integration of osmotin gene into genome of Rohida.
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