Development of a test system for simultaneous detection of HIV RNA and Hepatitis C virus (HCV) and DNA of Hepatitis B virus (HBV) in blood samples

Abstract

Background The aim of this work was to create a PCR test systems for simultaneous detection of HIV RNA, HCV RNA and DNA of HBV, which will have high sensitivity and high bandwidth. The issue of viral safety of blood products is of particular relevance in relation to the epidemiological situation with regard to viral hepatitis and HIV infection. The use of serological tests for screening of blood products has significantly reduced the risk of transmission of these infections in the transfusion. But from the moment of infection until the appearance of antibodies to the virus may be a considerable period, known as «serological window». In this situation, great care may have tests to detect the virus itself or its components. Using molecular genetics methods of diagnosis helped reduce the period of «serological window» and improve the safety of transfusion. The actual development of low-cost highly sensitive tests, allowing both to detect HIV and hepatitis viruses B and C, increase the capacity of blood transfusion stations in the molecular genetic studies. This involves the basic requirements for test systems for screening of donated blood the highest analytical sensitivity.