Abstract
Orf is a non-systemic, ubiquitous disease of sheep and goats caused by the orf virus (ORFV). ORFV occasionally causes cutaneous lesions in humans in contact with infected animals. In the present study, a real-time PCR method was established for detection of ORFV using the fluorescent chimeric dye SYBR Green I. Specific primers were designed to target a highly conserved region of the ORFV B2L gene. This method was able to detect a minimum of 20 copies of ORFV genomic DNA. The results showed no cross-reactions with other common DNA viruses. The time required for the test was approximately 1.5 h. Clinical test samples showed that this method was faster and had a higher sensitivity than traditional PCR. In conclusion, this novel, real-time PCR-based assay provides a rapid, sensitive, and specific method for ORFV detection. This test provides improved technical support for studies regarding the clinical diagnosis and epidemiology of ORFV.
Highlights
Orf, known as contagious ecthyma or scabby mouth, is a non-systemic, ubiquitous disease of sheep and goats caused by the orf virus (ORFV)
ORFV was added to the ovine fetal turbinate (OFTu) cells, and the cells were gently shaken for 10 min three times before the liquid was removed
In this study, we developed a sensitive and specific SYBR Green I real-time PCR assay to quantitatively detect ORFV
Summary
Known as contagious ecthyma or scabby mouth, is a non-systemic, ubiquitous disease of sheep and goats caused by the orf virus (ORFV). It occasionally causes cutaneous lesions in humans who contact infected animals. ORFV is a member of the Parapoxvirus genus and the Poxviridae family (Friebe et al 2011). The disease is characterized by pustular lesions around the mouth and nostrils. The mortality rate is low in adult animals, the morbidity rate is high. In immunosuppressed animals and in lambs, orf may be fatal (Gumbrell and McGregor 1997), with a reported mortality rate in lambs and kids of up to 10 and 93%, respectively (Hosamani et al 2007)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
