Development of a Stress Standard Base Criterion for Nerve Cell Injury by Hydrostatic Pressure Loading

Abstract

In this study, we try to develop a new head injury criterion in the automotive crash accident, which is evaluated by the hydrostatic pressure. Main subject of this study is to find the threshold value of the functional injury of the brain nerve cell, which is obtained by the impact loading experiment. PC-12 cultured nerve cell is employed to imitate the human's nerve cell and measure the critical pressure value for the occurrence of damage. We observed the cytoskeleton injury, and evaluated the decreasing ratio of fluoro-area caused by hydrostatic pressure loading. The disappearance area of fluorescence is caused by the creation of the actin rods and which means F-actin structural disorder. It reveals that the cytoskeleton injury depends on the hydrostatic pressure loading, and the injury threshold value, such as the critical pressure, is obtained. Finally, an availability of our new head injury criterion based on the nerve cell injury is confirmed.