Abstract
Daeshiho-tang (DSHT) is a traditional herbal formula consisting of six herbal medicines: Bupleurum falcatum L., Scutellaria baicalensis Georgi, Paonia lactiflora Pall., Pheum palmatum L., Poncirus trifoliata (L.) Raf., and Pinellia ternate (Thunb.) Makino. In this study, we developed a simultaneous analysis method based on high-performance liquid chromatography for the quality control of DSHT. Chromatographic separation of 10 marker components (gallic acid, albiflorin, paeoniflorin, naringin, benzoic acid, baicalin, poncirin, wogonoside, baicalein, and wogonin) was achieved using a water–acetonitrile system as the mobile phase with a SunFire C18 reversed-phase column. The developed analytical method was validated with respect to linearity, limit of detection, limit of quantitation, recovery, and precision. Among the 10 markers of DSHT in the established assay, baicalin, the main compound of Scutellaria baicalensis Georgi, was present in the highest concentration (36.86–46.17 mg/g). The validated assay will be useful for the quality control of DSHT.
Highlights
Traditional Korean medicine (TKM), traditional Chinese medicine (TCM), and Kampo medicine (KM) are attracting increasing interest as because their multicomponent and multitarget characteristics that enable the therapeutic or adjuvant treatment of a range of diseases [1]
To determine the optimal marker components for the quality assessment of DSHT, high-performance liquid chromatography (HPLC)–diode array detection (DAD) analysis was conducted on each component herbal medicine in DSHT to establish the major ingredients
Using the optimized HPLC–DAD assay, the 10 marker components in the DSHT sample were eluted with the resolution ≥2.13 and the retention factor ≥1.25 at 6.49, 16.45, 17.32, 21.11, 23.22, 26.32, 26.74, 29.98, 32.34, and 38.07 min, respectively (Figure 1)
Summary
Traditional Korean medicine (TKM), traditional Chinese medicine (TCM), and Kampo medicine (KM) are attracting increasing interest as because their multicomponent and multitarget characteristics that enable the therapeutic or adjuvant treatment of a range of diseases [1]. Iizuka et al presented HPLC profiling for DSHT [8], and Li et al performed a simultaneous analysis study on eight major components (paeoniflorin, naringin, sennoside A, baicalin, baicalein, saikosaponin A, rhein, and emodin) in DSHT [11]. These assays are limited by the long analysis time (90 min) required to separate the eight components and were primarily focused on establishing the conditions for the separation of isomers and profiling
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