Abstract
Development of a Simple Fluorescence Sensing System for Carbonyl Stress in Urine
Highlights
Carbonyl stress is caused by a series of active carbonyl compounds, including glyoxal, methylglyoxal, 3-deoxyglucosone, and acrolein
The most common method of detecting carbonyl stress is the detection of methylglyoxal, which has the highest carbonyl stress.[10]. High-performance liquid chromatography (HPLC) has been used to detect methylglyoxal.[11,12,13,14] it takes a long time to detect a sample, it is still an important detection method
The fluorescence values measured on the standard calibration curve of methylglyoxal with concentrations ranging from 0 to 560 μg/L and different dilution ratios of rat urine showed a linear relationship
Summary
Carbonyl stress is caused by a series of active carbonyl compounds, including glyoxal, methylglyoxal, 3-deoxyglucosone, and acrolein. Oxidative pressure is generated mainly through free radical changes in proteins and lipids, which accumulate into carbonyl stress.[1] When the concentration of carbonyls is very high, advanced glycation end products (AGEs) are formed. This process was originally discovered in the Maillard reaction.[2] From a pathological viewpoint, AGEs can modify the biological structure or interact with specific cell receptors to ISSN 0914-4935 © MYU K.K. https://myukk.org/. The fluorescent derivatization reagent 5,6-diamino-2,4hydroxypyrimidine sulfate dihydrate (DDP) is used to convert methylglyoxal to methyl-lumazine before detection.[14]
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