Abstract
A sensitive magnetic particles chemiluminescence enzyme immunoassay for determination of aflatoxin B1(AFB1) in corn was developed and validated. The method was developed based on a competitive immunoreaction format. Immunocomplex was formed through the competitive immunoreaction among AFB1 antigen, horseradish peroxidase-labeled AFB1 antigen and fluorescein isothiocyanate labeled anti-AFB1 monoclonal antibody. Immunocomplex combined with anti-fluorescein isothiocyanate (FITC) antibody coated magnetic particles. The magnetic particles served as both the solid phase and separator. The horseradish peroxidase(HRP)-luminol-hydrogen peroxide chemiluminescent system was chosen as the detection system. This method had a detection limit of 0.02 ng/mL and a linear range of 0.05-5 ng/mL. The intra-assay relative standard deviations were less than 9%. The inter-assay relative standard deviations were less than 15%. The recoveries were from 94.7% to 109%. The method has been successfully applied to the detection of aflatoxin B1(AFB1) in corn.
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