Abstract
As a commonly used turfgrass, Kentucky bluegrass (Poa pratensis L.) (KBG) has many commercially available cultivars for production. After several years of screening, two new lines were obtained (‘KBG03’ and ‘KBG04’), which have high tolerance to summer. The study showed that the two lines revealed similar morphological characteristics, with light green leaf color, narrow leaf blade, high plant height and light 1,000-grain weight. A total of 400 random amplified polymorphic DNA (RAPD) primers and 256 sequence-related amplified polymorphism (SRAP) primer combinations were screened among the two lines and other 4 imported commercial cultivars. The percentages of polymorphic sites were 65.5% (RAPD) and 22.6% (SRAP) respectively. By cluster analysis of RAPD and SRAP data, the dendrogram at a similarity of 0.29 gave two main clusters, of which one group had 4 commercial cultivars, and the other had the two new breeding lines. Furthermore, one specific band of ‘KBG04’ was successfully converted into a dominant sequence characterized amplified region marker (SCAR196). Then the SCAR marker was verified by 39 KBG DNA samples, including imported varieties, domestic varieties and self-breeding lines of our laboratory, and it exhibited high consistency with the original RAPD polymorphic amplification. The results showed that the SCAR marker can be used to distinguish the new line ‘KBG04’ from numerous KBG germplasms, which would be useful for cultivar identification and property rights protection in the future.
Highlights
Kentucky bluegrass (Poa pratensis L.) (KBG) is a cool-season, attractive truf grass widely used on home lawns, golf courses and athletic fields throughout temperate regions of the world (Beard, 1973)
Because the similar field visual quality of the two new lines, a combination of the two has been used, as a mixed population (KBG03+KBG04) registered as a new cultivar named ‘Huhe 2’, which was involved in the identification
Imported cultivars were planted and their seeds were harvested in Shanghai so that the data of their 1,000-grain weight could be more comparable with the domestic cultivars and lines
Summary
Kentucky bluegrass (Poa pratensis L.) (KBG) is a cool-season, attractive truf grass widely used on home lawns, golf courses and athletic fields throughout temperate regions of the world (Beard, 1973). This study was designed to identify the important agronomic traits and genome-specific molecular markers (SRAP and RAPD) of the new breeding lines. A SCAR marker was derived from the polymorphic band and tested in more KBG germplasms.
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