Abstract
The potential of SCAR marker for discrimination of a Thai jasmine rice (Oryza sativa L. cv. KDML 105) mutant, BKOS6, obtained from ion-beam-induced mutation, was evaluated. The improved rice variety, BKOS6, exhibited many remarkable characteristics which fitted the multiple cropping system characteristics of progressive agriculture including photoperiod insensitivity, early flowering, short in stature, and purple pigment accumulation in pericarp. The BKOS6 rice grain extract has already been proved that it exhibited higher antioxidant properties than the KDML 105 and other tested rice grain extracts. In this study, the BKOS6 specific SCAR marker was developed by HAT-RAPD analysis of rice genomic DNA. The marker was successfully used to identify BKOS6 variety and its hybrid varieties containing purple pigment accumulation in plant tissues. Moreover, it was found that this marker could be used to detect other purple pigmented rice varieties that genetically related to Thai jasmine rice. Recently, a wide variety of anthocyanin-based foods are believed to provide significant potential health benefits, and become more attractive. KDML 105 is also a Thai premier fragrant rice variety which is one of the main varieties of country’s rice export. Thus this molecular marker could be useful for commercial and breeding purposes of BKOS6 mutant and other developed varieties from KDML 105 which contain anthocyanin accumulation.
Highlights
Increased grain yield and improved rice quality are absolutely necessary to feed the world’s population and to improve its nutrition
The re-ion bombarded mutants, obtained from re-ion bombardment of BKOS6 seeds, which exhibit purple pigment accumulation in their tissues, were detectable by the sequence characterized amplified regions (SCARs) marker. These results indicate that the SCAR marker might associate with purple color trait in rice lines which have been developed from KDML 105
Due to many remarkable improved characteristics of BKOS6 variety, a specific SCAR marker was generated for its molecular identification
Summary
Increased grain yield and improved rice quality are absolutely necessary to feed the world’s population and to improve its nutrition. Thai jasmine rice seeds bombarded with nitrogen ions revealed one of new rice varieties, named BKOS6 rice, showing short stature (at 49 cm of the M1 plant height in culm length on the harvest day), photoperiod insensitivity, early flowering and accumulation of purple pigment in various tissues [1]. This variety showed mutational stability in all characteristics when observed in M1-M8 cultivations [2]. It was found that there was no significant difference between the antioxidant properties of the cooked and uncooked BKOS6 rice
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