Abstract
A rapid and simple ribotyping method for Corynebacterium diphtheriae was developed, based on 16S–23S rRNA spacer region analysis, by using polymerase chain reaction (PCR)–single strand conformation polymorphism (SSCP). All 123 strains tested were PCR positive with primer set rG1 and rL1, and each reaction resulted in only one amplicon of approximately 440 bp in size. When 19 ribotyping type strains and 26 previously ribotyped strains were analyzed by this method, 29 distinct SSCP patterns were identified. Seventeen of 19 established ribotypes could easily be differentiated, and in some cases SSCP ribotyping provided even better resolution than traditional ribotyping. This method provides a rapid, reliable, and reproducible alternative for the traditional ribotyping, which can significantly aid in epidemiological studies, especially where large numbers of strains need to be rapidly analyzed.
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