Abstract
A new, rapid and efficient reverse phase Liquid Chromatography (RP-LC) method was developed for determination of Gibberellin A4 (GA4) in samples of flower stalk of Dasylirion cedrosanum and vegetative tissue of Epithelantha micromeris. Purification of GA4 was carried out by solid phase extraction (SPE), in Epithelantha micromeris. In the chromatography method was obtaining a retention time of 2.1 min, using Hypersil GOLD C-18 column (100 × 4.6 mm dim and size particle 5 μ), mobile phase 50/50 acetonitrile/water and a flow 1.0 ml/min. Detection was carried out by a UV detector set at 205 nm, and a quantization limit of 0.4 mg/L. The obtained correlation coefficient was 0.995.
Highlights
Gibberellins are an important group of isoprenoid phytohormones that occur in small amounts in higher plants
The quantitative analysis of gibberellins in plant tissues is difficult because these phytohormones are present in low concentrations and the tissues are very rich in interfering substances, especially secondary metabolites [7]
The chromatograph used was a Spectra System Thermo Scientific equipped with solvent degasser (SCM 1000), a quaternary pump (P4000) coupled to a manual injector with a 20 μl fixed loop, VWD Ultra violet detector (UV 1000) and reverse phase Hypersil GOLD C-18 column (100 × 4.6 mm dim and size particle 5 μ)
Summary
Gibberellins are an important group of isoprenoid phytohormones that occur in small amounts in higher plants. They are involved in the development and regulation of different growth processes throughout the life-cycle of plants; each gibberellin (GA) affects a different part of the plants. The most important bioactive gibberellins are GA1, GA3, GA4 and GA7 [3]. Numerous reports have shown gibberellins (GAs) play a role in the growth, development and sexual differentiation processes of different plant species [6]. The quantitative analysis of gibberellins in plant tissues is difficult because these phytohormones are present in low concentrations and the tissues are very rich in interfering substances, especially secondary metabolites [7] [8] In studies made in Satika spruce (Picea stichenis), it was concluded that the separation of GA4 in particular was not achieved; quantification was not done properly, probably due to a combination of low GA4 and a high amount of interfering substances such as resin acids [9]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
