Abstract
The Giant Gartersnake (Thamnophis gigas) is a low density visually evasive species with a low detection probability based on standard field survey methods (e.g., traps, visual census). Habitat loss has resulted in extirpations or serious declines for T. gigas populations throughout the southern two thirds of its historic range. Uncertainty regarding its current distribution and occupancy present management challenges for the species. Enhancing survey sensitivity through development of environmental DNA sampling (eDNA) methods would improve compliance monitoring under the Endangered Species Act, recovery planning for T. gigas, and evaluation of California’s Central Valley tule marsh habitat on which this species depends. To address these needs, we designed and validated diagnostic quantitative Polymerase Chain Reaction (qPCR) assays for identifying portions of the Cytochrome B (CytB) and the Nicotinamide adenine dinucleotide (NADH) dehydrogenase subunit 4 (ND4) genes of the T. gigas mitochondrial genome. The designed ND4 qPCR assay was not specific to T. gigas DNA and amplified DNA from a closely related and spatially co-occurring Thamnophis species (T.s. fitchi). The CytB T. gigas qPCR assay proved specific to a species level with a sensitivity that reliably detected T. gigas DNA at a concentration of 2.0x10-5 ng μL-1. To assess detection range, coordinated field sampling was conducted at aquatic sites with an observed and documented population of T. gigas. The T. gigas qPCR assay reliably detected DNA from samples taken 300m downstream from the known source. We then used environmental eDNA sampling and qPCR analysis to augment unsuccessful trap surveys in the southern range of T. gigas and detected DNA in 28 of the 52 locations sampled, confirming that T. gigas was still present at some sites where physical trapping failed to identify presence. QPCR-based DNA detection coupled with eDNA sampling methods provides an effective means to obtain critical population metrics from this otherwise cryptic, federally protected and hard to study organism, offering great promise for elucidating patterns of occupancy with greater efficiency and at far less cost than trapping methods, particularly where detection probabilities are low.
Highlights
The Giant Gartersnake (Thamnophis gigas) is a federally and state threatened species endemic to California’s Central Valley
BLAST results evaluating the 687 bp consensus Cytochrome B (CytB) fragment and the 684 bp ND4 fragment were evaluated by Integrated DNA Technologies (IDT) scientific applications support team for quantitative Polymerase Chain Reaction (qPCR) assay design incorporating PrimeTime1 LNA1 (Locked Nucleic Acid) qPCR 5’ probes
From the 1940s through the 1970s, T. gigas in the San Joaquin Valley occurred at densities facilitating routine observations through visual encounter surveys (e.g., [1,31])
Summary
The Giant Gartersnake (Thamnophis gigas) is a federally and state threatened species endemic to California’s Central Valley. Described as among California’s most aquatic gartersnakes [1], T. gigas is associated with low gradient streams, valley floor wetlands, and marshes. The species requires wetlands to forage for prey (i.e. fish and amphibians), upland areas for basking, upland burrows as summer shelter, and higher elevation uplands for winter brumation [2,3,4,5]. T. gigas typically emerges in March, is active (foraging and breeding) through spring and summer, and seeks winter refuge in the fall [5,6,7,8,9]. T. gigas has been historically known to associate with marshes, ponds and low-gradient streams. Identifying when and where T. gigas occupy native habitat is a fundamental first step in any recovery action
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.