Abstract
ABSTRACT A competitive lateral flow immunoassay (LFA) using fluorescent microspheres (FMs) as label was developed for the quantitative detection of chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) in milk. The limit of detection (LOD) for CAP, TAP and FF in milk was 0.08, 0.8 and 1.9 μg L−1, respectively. The recovery of intra and inter-assay ranged from 87.7% to 107.0% and 82.5% to 112.3%, with corresponding coefficient of variations less than 11.3% and 14.5%, respectively. The sensitivity of the FMs lateral flow assay (FMs-LFA) was comparable to those of the colloidal gold lateral flow assay. A quantitative comparison of the FMs-LFA and LC–MS/MS analysis of milk samples indicated good agreement between the two methods. The FMs-LFA can be used as a reliable, rapid and cost-effective method for food safety analysis.
Highlights
Chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) are broad-spectrum antibiotics which have been extensively used to treat various infections in animals
In China, CAP was prohibited for use in any food-producing animals and the maximum residue limit for TAP was set at 50 μg L−1 in milk
For fluorescent microspheres (FMs)-lateral flow immunoassay (LFA), the average intra-assay recovery values of CAP, TAP and FF ranged from 87.7% to 107.0%, with coefficient of variations (CVs) less than 11.3%, the average inter-assay recovery values of CAP, TAP and FF ranged from 82.5% to 112.3%, with CV less than 14.5%
Summary
Chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) are broad-spectrum antibiotics which have been extensively used to treat various infections in animals. In China, CAP was prohibited for use in any food-producing animals and the maximum residue limit for TAP was set at 50 μg L−1 in milk. A number of analytical methods have been developed to detect CAP, TAP and FF; these include high-performance liquid chromatography (Xie et al, 2011), gas chromatographymass spectrometry (GC-MS) (Shen et al, 2009), liquid chromatography tandem mass spectrometry (LC–MS/MS) (Barreto, Ribeiro, Hoff, & Dalla Costa, 2016; Li, Hu, Huo, & Xu, 2006; Rezende, Filho, & Rocha, 2012), enzyme-linked immunosorbent assay (Fodey, Murilla, Cannavan, & Elliott, 2007; Liu et al, 2014; Xu, Xu, Ma, et al, 2015) and lateral flow immunoassay (LFA) (Bai et al, 2013; Guo et al, 2015; Samsonova, Cannavan, & Elliott, 2012; Xu, Xu, Ma, Liu, et al, 2015). The LFA has proven to be a suitable tool for onsite test to detect various types of analytes (Hsieh et al, 2013)
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