Abstract
A quality assurance (QA) procedure is developed that enables analysts to effectively test the intrinsic activity of a SERS substrate - silver colloids - before applying them to a precious art sample. SERS measurements of adsorbed citrate or alizarin should not be used to establish substrate fidelity. Instead, we recommend carmine and madder lake pigments as first steps in QA. After colloid activity is established, integrating the QA protocol with sample pretreatment strategies provides for the reproducible identification of unknown colorants in microscopic samples from references and an actual painting.
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