Abstract
A recombinant positive control plasmid was developed to be used in PCR for Orientia tsutsugamushi. This pUC19-based plasmid contains the O. tsutsugamushi DNA sequence, part of which is replaced with Candida albicans DNA. pUC19-Posi is a PCR control that provides several advantages over currently used positive controls for both universal and serotypical PCR.
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