Abstract

A batch UV reactor system was constructed to determine the UV inactivation kinetics of aerosolized Bacillus subtilis spores. This reactor consisted of a small-scale cylindrical reactor placed in a collimated beam system. The average fluence rate inside the reactor was determined using the same standard protocol as that used in collimated beam UV inactivation studies of waterborne microorganisms, with some modifications. A multi-target model was used to model the observed inactivation versus fluence (UV dose) for aerosolize spores. A statistical comparison of the kinetic models showed that there was no significant difference between the rate constants of the aerosolized and waterborne spores. The results also indicated that, within the relative humidity range investigated (50–83%) the relative humidity does not affect the UV inactivation rate of the spores. The experimental methodology demonstrated in this article can be used to generate the UV inactivation kinetic information necessary for the rational de...

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