Abstract

Shoots of Citrus sp. Kuharske were used to develop protocols for rooting reportedly HLB resistance rootstocks under intermittent mist. Investigated were shoot maturity, nodes per cutting, leaves per cutting, effects of buds, auxin concentrations and auxin solvent. Shoot maturity was most influential for success, with cuttings taken below the first 30 cm of active terminal growth producing greater root generation. Use of a thickening agent (Natrosal) to dilute the commercial auxin was second most in importance for rooting success. Root mass increased with increasing number of leaves. Cutting stems between nodes or below the lowest bud were inconsequential. To produce maximum number of viable cuttings, single node-single leaf cuttings were preferred. Single bud cuttings produced one shoot after rooting. This was adventitious since multi-node cuttings usually sprouted new shoots that would need to be removed before budded. Evaluation of the best combination of auxin and cutting-related attributes were evaluated with four additional common rootstocks in June 2016. Rooting was 100% successful. A quick dip (0.5 s) in a 7500 ppm solution of Dip&Gro produced the most root generation in six weeks for all rootstocks. Root quantity varied by rootstock.

Highlights

  • Florida’s citrus industry has been devastated by Huanglongbing (HLB) disease, known as citrus greening

  • Shoot maturity was most influential for success, with cuttings taken below the first 30 cm of active terminal growth producing greater root generation

  • Siaticus [1], HLB is spread by Asian psyllids and progressively attacks and kills fine root

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Summary

Introduction

Florida’s citrus industry has been devastated by Huanglongbing (HLB) disease, known as citrus greening. Up to 40% loss of fine roots occurs before symptoms in above ground portions of trees are noticeable. Infection leads to smaller fruit size and loss, significant yield loss, and over time dying and unproductive groves. Common citrus rootstocks used for decades are susceptible to HLB

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