Abstract
This paper presents an analytical procedure for the determination of folic acid employing commutation flow analysis process and detection by chemiluminescence. The procedure is based on the reaction of hexacyanoferrate(III) with folic acid, followed of luminol oxidizing reaction in alkaline medium, resulting in emission of radiation at 425 nm. After optimization the experimental variables, the proposed procedure afforded the following useful features. A linear response ranging from 0.1 to 1.00 mg mL-1 folic acid (R2= 0.993), a detection limit (3σ criterion) 0.046 mg mL-1, a sampling rate of 156 determination per hour, a relative standard deviation less than 0.5% (n = 6) for a 0.6 mg mL->1 folic acid standard solution and a waste generation of 0.86 mL per determination.
Highlights
IntroductionFolic acid (N-[p{[(2-amino-4-hydroxy-6-pteridinyl) methyl]amino}benzoyl]-L-glutamic acid, is a water soluble vitamin belonging to the B complex (vitamin B9 or vitamin M),[1,2] which is produced by plants (green leaves, algae) and micro-organisms.[3,4,5,6] Several papers has been pointed out that folic acid contribute to the formation of red blood cells, being identified as an anti-anemia and growth factor.[3,7,8] This compound participates in the synthesis of DNA bases and chains, being required for the formation of new cells, mainly in condition of rapid cell division and growth, including pregnancy and infancy.[9]
Folic acid (N-[p{[(2-amino-4-hydroxy-6-pteridinyl) methyl]amino}benzoyl]-L-glutamic acid, is a water soluble vitamin belonging to the B complex,[1,2] which is produced by plants and micro-organisms.[3,4,5,6]
The analytical procedure was based on the reaction of folic acid with hexacyanoferrate(III) and the remaining oxidant reacts with luminol in alkaline medium, resulting in emission of electromagnetic radiation
Summary
Folic acid (N-[p{[(2-amino-4-hydroxy-6-pteridinyl) methyl]amino}benzoyl]-L-glutamic acid, is a water soluble vitamin belonging to the B complex (vitamin B9 or vitamin M),[1,2] which is produced by plants (green leaves, algae) and micro-organisms.[3,4,5,6] Several papers has been pointed out that folic acid contribute to the formation of red blood cells, being identified as an anti-anemia and growth factor.[3,7,8] This compound participates in the synthesis of DNA bases and chains, being required for the formation of new cells, mainly in condition of rapid cell division and growth, including pregnancy and infancy.[9]. The amount of folic acid in pharmaceuticals, allows that solutions can be prepared at appropriate concentration, allowing that the analyte determination would be formed, employing a very simple radiation detection instrument based on a photodiode. The MCFA process, when implemented employing solenoid mini-pump for fluid propulsion, afforded facilities to meet the GAC requisite, concerning to reduction of waste generation.[18,19] This feature is exploited in this work to develop an automated analytical procedure environmentally friendly for the determination of folic acid in pharmaceuticals. In the second step (St2), mini-pumps P2 and P4 were switched on/off at the same time When this happen, the mix comprising sample and hexacyanoferrate(III) solution, displaced by the carrier fluid (Cs), merged into the joint device (x2) with aliquots of luminol solution. The magnitude of the generated signal presents a linear relationship with intensity of the radiation generated when luminol was oxidized by the hexacyanoferrate(III) ions
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