Abstract
A simple and reproducible short-term fish cell culture technique with minimum or no stress to the specimens is described. Gill, scale and fin tissues from eight fish species viz., Labeo rohita, Catla catla, Cirrhinus mrigala, Cyprinus carpio, Clarias batrachus, Heteropneustes fossilis, Anabas testudineus and Oreochromis mossambicus, were used to develop primary cell cultures. A primary culture from the caudal fin of L. rohita was successfully obtained on the fourth post-culture day using L-15 medium supplemented with 20% Fetal Calf Serum (FCS) and 20% Fish Muscle Extract (FME) at an incubation temperature of 28-29°C. This experiment could not obtain primary cultures from other fish species and tissues, either due to non-attachment of explants or fungal contamination.
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