Abstract
Nucleic acid detection is of great significance in clinical diagnosis, environmental monitoring and food safety. Compared with the traditional nucleic acid amplification detection method, surface plasmon resonance (SPR) sensing technology has the advantages of being label-free, having simple operation, and providing real-time detection. However, the angle scanning system in many SPR angle modulation detection applications usually requires a high-resolution stepper motor and complex mechanical structure to adjust the angle. In this paper, a portable multi-angle scanning SPR sensor was designed. The sensor only uses one stepping motor to rotate a belt, and the belt pulls the mechanical linkages of incident light and reflected light to move in opposite directions for achieving the SPR angle scanning mode that keeps the incident angle and reflected angle equal. The sensor has an angle scanning accuracy of 0.002°, response sensitivity of 3.72 × 10−6 RIU (refractive index unit), and an angle scanning range of 30°–74°. The overall size of the system is only 480 mm × 150 mm × 180 mm. The portable SPR sensor was used to detect nucleic acid hybridization on a gold film chip modified with bovine serum albumin (BSA). The result revealed that the sensor had high sensitivity and fast response, and could successfully accomplish the hybridization detection of target DNA solution of 0.01 μmol/mL.
Highlights
Nucleic acid is the basic genetic material of creatures and plays an extremely significant role in the growth, reproduction and inheritance of creatures [1,2]
Compared with the mentioned techniques, nucleic acid detection based on surface plasmon resonance (SPR) sensing technology is a label-free, real-time, simple to operate, and highly sensitive detection method [11,12,13,14,15]
The angle scanning test of the SPR sensor developed in this paper was performed
Summary
Nucleic acid is the basic genetic material of creatures and plays an extremely significant role in the growth, reproduction and inheritance of creatures [1,2]. Including polymerase chain reaction (PCR) and other isothermal amplification technologies, DNA microarray technologies are conventional methods for nucleic acid detection [6,7,8]. The detection method based on PCR is time-consuming and complex to operate. DNA microarray technology is stringent for technical personnel It requires expensive instruments and complex preparation of samples. Droplet digital PCR is a new method developed on the basis of conventional PCR. It has the advantages of absolute quantification and high sensitivity. Compared with the mentioned techniques, nucleic acid detection based on surface plasmon resonance (SPR) sensing technology is a label-free, real-time, simple to operate, and highly sensitive detection method [11,12,13,14,15]. SPR sensing technology has been already used to study nucleic acid interactions
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
