Abstract
该工作报道了一种自行设计研制的便携式微型液相色谱仪(portable micro liquid chromatograph, p-μLC)。p-μLC集成了二元大推力注射泵作为流动相驱动装置、毛细管整体柱为分离介质和紫外-可见/荧光两用流通池为在线检测单元。自行设计研制的二元大推力注射泵可以实现等度/梯度洗脱和流动相再装填功能,可控流速范围在0.025 μL/min到5.6 mL/min之间;自制的甲基丙烯酸酯C-18有机聚合物毛细管整体微柱可实现自有机小分子至生物大分子的分离;自行研制的光纤式紫外-可见/荧光两用流通池,可以通过光纤导入来自光源的紫外光和可见光,并采集透射光和与入射光反方向射出的荧光信号,流通池内使用自聚焦透镜和全反射光导毛细管等器件提高通光效率和吸收光程;两用流通池通过光纤分别连接由大功率发光二极管/脉冲氙灯光源和微型光栅光谱仪所组成的检测装置进行在线吸收和荧光光谱检测,检测波长范围为220~700 nm。p-μLC采用整体手提箱式结构,流路模块、检测模块等位于下主箱体中,采集、控制模块等位于上盖中,全重不超过8 kg。仪器由装载了自编控制采集软件的内置平板电脑进行控制和数据采集。使用自行制备的甲基丙烯酸酯C-18有机聚合物毛细管整体柱,在等度洗脱模式下,在p-μLC上分离了烷基苯化合物混合样品,其分离检测效果可以与商品化大型HPLC仪器相媲美。
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
