Development of a Poly(Lactic Acid) and Fibrin Device for Use in Bone Regenerative Medicine

Abstract

Bone regenerative medicine aims to overcome the limitations of the conventional treatments for bone repair in attempt to develop therapeutic strategies based on biomaterials capable of acting as temporary substitutes for guiding and stimulating the tissue repair, in order to reduce or eliminate the need for autologous donor tissues. The aim of this study was develop a Poly(lactic acid) (PLA) and fibrin cellularized bioactive scaffold to establish a new strategy with potential to use in bone regenerative medicine. For this purpose, three‐dimensional PLA scaffolds were initialy produced by Fused Deposition Modeling (FDM) 3D printing technique, using computer aided drawing (CAD) software. These scaffolds were embedded in the suspension containing human pure platelet‐rich plasma (hP‐PRP) and human infrapatellar fat pad derived mesenchymal stem cells. Then, the platelets were exogenously activated by thrombin and calcium chloride, leading the fibrin to polymerize and form a cellularized device. The scaffolds were maintained in serum‐free Dulbecco's Modified Eagle Medium for 5 days. Scanning electron microscopy and fluorescence microscopy demonstrated that cells kept viable and in tree‐dimensional conformation during the experimental period of time. The results suggest that PLA scaffolds, associated with hP‐PRP fibrin matrix, generated a rigid bioactive scaffold capable of mantain cellular viability and represent a promising strategy for bone regenerative medicine.Support or Funding InformationFUNADESP‐ Fundação Nacional de Desenvolvimento do Ensino Superior Particular