Abstract
We have developed and characterized a novel perfusion bioreactor system which facilitates the observation of living cells for extended periods of time. Cell viability showed that cell growth rate cultured via the novel flow perfusion chamber system revealed a similar trend to that of static culture. Also, The result of cell viability under the static culture and flow perfusion culture via the developed bioreactor system showed that cell growth rate was significantly higher by the flow perfusion culture than by static culture conditions. The cell growth was efficiently increased to about 10 - 15% by perfusion culture conditions. The developed chamber system has been successfully used to maintain and record cultured alveolar bone marrow stem cells. It was possible to monitor and capture a live cell morphology cultured in the perfusion bioreactor.
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