Abstract
Heme plays a vital role in cell biology and dysregulation of heme levels is implicated in a wide range of diseases. However, monitoring heme levels in biological systems is currently not straightforward. A short synthetic peptide probe containing 7-azatryptophan is shown to bind hemin in vitro with quenching of the azatryptophan fluorescence. This chemical tool can be used to detect the change in free heme induced in human skin cells upon exposure to UVA irradiation.
Highlights
Heme plays a vital role in cell biology and dysregulation of heme levels is implicated in a wide range of diseases
The total heme content of the cell comprises the mostly inert heme that is tightly associated with hemoproteins such as cytochromes and the free heme pool which is available for regulatory protein binding and signalling
Questions remain over the concentration of the free heme pool, cellular distribution, oxidation state and dynamics – how it is controlled and how it responds to different stimuli
Summary
Heme plays a vital role in cell biology and dysregulation of heme levels is implicated in a wide range of diseases. Development of a peptide-based fluorescent probe for biological heme monitoring† A short synthetic peptide probe containing 7-azatryptophan is shown to bind hemin in vitro with quenching of the azatryptophan fluorescence.
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