Development of a pea protein‐/chitosan‐based bioactive film using Aronia melanocarpa polyphenols as a bioactive ingredient

Abstract

The study was designed to develop a pea protein- (PP) and chitosan (CS)-based bioactive composite film using purified Aronia melanocarpa polyphenols (AMP) as a bioactive ingredient (PP-CS-AMP). First, AMP was extracted and purified, and the antioxidant properties of AMP were tested, and the result indicated that AMP showed stronger antioxidant properties than vitamin C (Vc). Second, five kinds of PP-CS film were developed: PP including unextruded pea protein (NEP), extrusion pea protein (EP), papain co-extruded pea protein (PEP), neutral protease co-extruded pea protein (NPEP), and double enzyme combined extrusion pea protein (DCEP). The five kinds of PP-CS film were characterized and evaluated for various functions and properties; principal component analysis and membership evaluation were used to analyze the comprehensive scores of the films. It was observed that the NPEP-CS film obtained the highest score. Extrusion and enzyme extrusion treatments improved the structure and properties of PP, and therefore enhanced the properties of PP-CS film. Finally, NPEP-CS-AMP film was developed (AMP content 0, 4.0% and 8.0%, w/v) and tested for their performance, and the results suggested that the addition of 4.0% AMP gives the NPEP-CS-AMP film excellent physical and antioxidant properties. Novelty impact statement Aronia melanocarpa polyphenols (AMP), as a bioactivity ingredient was firstly incorporated into pea protein (PP) - chitosan (CS) film. Extrusion and enzyme extrusion can improve the structure and properties of PP, which is beneficial to improvement on the properties of PP-CS film. NPEP-CS-AMP film exhibit excellent physical properties, structural performance and high antioxidant activity.