Abstract
Diabetes is one of the top 10 global causes of death. About one in 11 global adults have diabetes. As the disease progresses, the mortality rate increases, and complications can develop. Thus, early detection and effective management of diabetes are especially important. Herein, we present a novel glycated human serum albumin (GHSA) aptamer, i.e., GABAS-01, which has high affinity and specificity. The aptamer was selected by reduced graphene oxide-based systematic evolution of ligands by exponential enrichement (rGO-based SELEX) against GHSA. After five rounds of selection through gradually harsher conditions, GABAS-01 with high affinity and specificity for the target was obtained. GABAS-01 was labeled by FAM at the 5′-end and characterized by measuring the recovery of a fluorescence signal that is the result of fluorescence quenching effect of rGO. As a result, GABAS-01 had low-nanomolar Kd values of 1.748 ± 0.227 nM and showed a low limit of detection of 16.40 μg/mL against GHSA. This result shows the potential application of GABAS-01 as an effective on-site detection probe of GHSA. In addition, these properties of GABAS-01 are expected to contribute to detection of GHSA in diagnostic fields.
Highlights
Diabetes is a group of metabolic diseases in which high blood sugar level persists for a long time
We propose a novel sequence of DNA aptamers with high binding affinity for glycated human serum albumin (GHSA) and suggest that the screening method is important for constructing the sensor system
The non-bound sequences on reduced graphene oxide (rGO) and the bound sequences with the counter target were separated by centrifugation and removed
Summary
Diabetes is a group of metabolic diseases in which high blood sugar level persists for a long time. Insulin secretion is blocked, and insulin action is hindered when sugar builds up in the bloodstream. Type 2 diabetes is the more common type of diabetes and is caused by lifestyle and genetics [3,4]. In this type, cells develop a resistance to the action of insulin to result in impaired insulin secretion. As the pancreas does make not enough insulin to overcome this resistance, insulin actions are relatively reduced, and the blood sugar level is increased [4,5,6]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
