Abstract
A therapeutic strategy to improve wound healing has become an increasingly important medical task due to the rising incidence of adiposity and type II diabetes as well as the proceeding population aging. In order to cope with the resulting burdens, new strategies to achieve rapid and complete wound healing must now be developed. Accordingly, the development of a bioactive wound dressing in the form of a messengerRNA (mRNA)-bearing poly(lactide-co-glycolide acid) (PLGA) coating on surgical suture is being pushed further with this study. Furthermore, the evaluation of the polymer-based transfection reagent Viromer RED has shown that it can be used for the transfection of eukaryotic cells: The mRNA gets properly complexed and translated into a functional protein. In addition, the mRNA-PLGA coating triggered the expression of the keratinocyte growth factor (KGF) in HaCat cells although KGF is not expressed under physiological conditions. Moreover, transfection via surgical sutures coated with mRNA does not affect the cell viability and a proinflammatory reaction in the transfected cells is not induced. These properties make the mRNA-PLGA coating very attractive for the in vivo application. For the future, this could mean that through the use of mRNA-coated sutures in surgical wound closure, cells in the wound area can be transfected directly, thus accelerating and improving wound healing.
Highlights
In industrialized nations, the incidence of obesity and type II diabetes continues to increase as society simultaneously grows older [1]
Most importantly, establishing a mRNA coating based on the biodegradable polymer poly(lactide-co-glycolide acid) (PLGA) with Viromer RED used as transfection reagent has been successful: HEK293 cells, HaCat cells, and primary fibroblasts could be transfected by coated seam material, and the mRNA-PLGA coating even triggered the expression of keratinocyte growth factor (KGF) in HaCat cells, they do not form this growth factor under physiological conditions [27]
Our work describes a novel approach to support the process of wound healing by mRNA-coated suture materials
Summary
The incidence of obesity and type II diabetes continues to increase as society simultaneously grows older [1]. In Germany alone, 2.5% of the population suffers from chronic wounds with rising tendencies [2]. Chronic wounds are, on the one hand, a major burden for the person concerned and, on the other hand, they pose a socio-economic challenge: About 2% of the health budget must be raised each year to treat poorly healing wounds [3]. In order to cope with the enormous financial burden, new strategies must be developed for achieving rapid and complete wound healing. Wound healing is a complex three-stage process mediated by a large number of molecules such as different enzymes, cytokines, and growth factors [4]. Surgical sutures are used for wound closure to achieve this state whenever tissue separation occurs due to an incision, puncture, abrasion, or other injuries [6]
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