Abstract
We have developed a novel optical bionanosensor platform using a supported bilayer lipid membrane (SBLM), which has generic multi-analyte sensing capabilities. The SBLM is produced using a novel combination of ordered nanostructured thin film i.e. Langmuir–Blodgett (LB) and self-assembly deposition methodologies. A heptamer linear RGD (arginine–glycine–aspartate) containing peptide was covalently attached to a BODIPY (donor) lipid dye and utilised as an optical biosensor for integrin α vβ 3 loaded HUVEC’s (sensitivity=1000 cells ml −1). A second BODIPY (acceptor) lipid dye was integrated into the SBLM thus enabling signal amplification via a Forster resonance energy transfer (FRET) mechanism. The fluidity of the bilayer was confirmed via fluorescence recovery after photobleaching (FRAP) techniques and was performed without the need for fusogens.
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