Abstract
Camelid antibodies are composed of two heavy chains but lack the CH1 domain. These antibodies contain a single variable domain (VHH) and two constant domains (CH1 and CH3). Alkaline phosphatase is one of the most extensively used immunodiagnostic reagents for medical and research application because of its homodimer formation. The aim of this study was to develop a novel stable single chain nanobody library against alkaline phosphatase that can have therapeutic and diagnostic use. Here, we are reporting first time the construction of recombinant antibody of phage display technology in India. After immunization with alkaline phosphatase enzyme and adjuvant in Camelus dromedarius, single domain heavy chain antibody was generated from V-D-J H gene arrangement in B-cell. We developed novel protocol for the production of stable single-domain antibodies in the camelid format. We believe that camelid antibodies will be a method of choice to control immunity related diseases and infectious disease.
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