Abstract
In this study, we reported the development of Prussian blue (PB), poly(pyrrole-2-carboxylic acid) (PPCA), and glucose oxidase (GOx) biocomposite modified graphite rod (GR) electrode as a potential biocathode for single enzyme biofuel cell fuelled by glucose. In order to design the biocathode, the GR electrode was coated with a composite of PB particles embedded in the PPCA shell and an additional layer of PPCA by cyclic voltammetry. Meanwhile, GOx molecules were covalently attached to the carboxyl groups of PPCA by an amide bond. The optimal conditions for the biocathode preparation were elaborated experimentally. After optimization, the developed biocathode showed excellent electrocatalytic activity toward the reduction of H2O2 formed during GOx catalyzed glucose oxidation at a low potential of 0.1 V vs Ag/AgCl, as well as good electrochemical performance. An electrocatalytic current density of 31.68 ± 2.70 μA/cm2 and open-circuit potential (OCP) of 293.34 ± 15.70 mV in O2-saturated 10 mM glucose solution at pH 6.0 were recorded. A maximal OCP of 430.15 ± 15.10 mV was recorded at 98.86 mM of glucose. In addition, the biocathode showed good operational stability, maintaining 95.53 ± 0.15% of the initial response after 14 days. These results suggest that this simply designed biocathode can be applied to the construction of a glucose-powered single enzyme biofuel cell.
Highlights
Body temperature, pH, and salt concentration, as well as be able to generate a sufficient amount of energy and have good operational stability
cyclic voltammetry (CV) was used for the electrochemical synthesis of the composite consisting of Prussian blue (PB) particles embedded in a PPCA shell (PB-PPCA) and for the formation of an additional PPCA layer over particles embedded in the PPCA shell (PB-PPCA) (PB-PPCA/PPCA)
The operation of the biocathode can be explained by the electrocatalytic activity of PB towards to the reduction of H2O2 formed during glucose oxidase (GOx) catalyzed oxidation of glucose
Summary
Body temperature, pH, and salt concentration, as well as be able to generate a sufficient amount of energy and have good operational stability. The performance of EBFCs has greatly improved by using various nanomaterials, such as carbon nanotubes (CNTs), graphene oxide (GO), noble metal nanoparticles or conjugated polymers (CPs) These materials often have good biocompatibility, electrical conductivity and large surface area. Biocathodes based on immobilized peroxidase (PO)[14,22] and biocathodes in which GOx is combined with PO that catalyses the reduction of H 2O2, produced during glucose oxidation on GOx modified e lectrodes[23], have been published Such systems have a drawback: the use of two enzymes, which makes the system more complex and increases the cost.
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