Abstract
Brucellosis is a highly contagious disease that affects public health and wildlife species worldwide. The efficient determination is required to reduce the transmission of infection in human and animals. In this research, a new nano-biosensor was developed for diagnosis of Brucella abortus (B. abortus) bacteria based on graphene quantum nanoparticles (GQNPs), magnetic nanoparticles (MNPs) and polyclonal antibody. First, GQNPs and MNPs were synthesized and conjugated with B. abortus polyclonal antibody (named here IgG1 and IgG2, respectively). The morphology and size of the synthesized nanoparticles were characterized using SEM, DLS and FTIR. Then, the GQNP-IgG1 and MNP-IgG2 conjugates were added to B. abortus to form a sandwich structure. After isolating the sandwiches by a magnet, the detection method was approved successfully using a fluorescence spectrophotometer as a quick and precise procedure for detection of B. abortus.
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