Abstract
A capillary immunochromatographic assay (CICA) using colloidal gold-labeled monoclonal antibodies was developed for semi-quantitative detection of clenbuterol (CLE) in this paper. This novel system fabricated in a competitive format was supported by glass capillary because of its easily modified, low cost, favorable optical properties. Here, two different-sized gold nanoparticles (13 and 26 nm) were prepared and corresponding assay conditions were optimized. By comparing the results between the two different-sized AuNPs, the 26-nm-AuNPs manifested higher sensitivities which the detection limit was estimated to be 0.35 ng/ml and the cut-off level with the naked eye of 2.5 ng/ml was observed. Using the optimized parameters, the intra-assay and inter-assay coefficients of variation (CVs) were ≤10.2 % and the recovery reached 70.8–115.5 % in pig urine, pork, beef, pork sausage and luncheon meat. The performance, including sample preparation, could be accomplished within 24 min. These results suggest that CICA holds promising use for sensitive, simple and low-cost on-site detection of CLE.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
