Abstract
AbstractA multiresidue high performance liquid chromatography procedure with electrochemical detection (amperometric) (HPLC‐ECD) using a C18 narrow bore column was fully optimized for the separation of a standard mixture of macrolides. Up to five macrolides, josamycin (JOS), kitasamycin (KIT), rosamicin (ROS) roxithromycin (ROX) and oleandomycin (OLE), the latter being selected as internal standard for the quantification, were properly separated using an isocratic elution mode. The influence of classical chromatographic and instrumental parameters was investigated in order to optimize them before the calibration assay. The method was succesfully applied to the determination of ROS and ROX in human urine.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
