Abstract
To identify the bacteria of the Yersinia genus and pathogenic species (Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica) in a single reaction, a multiplex PCR technique, which uses genes of nonspecific porins (OmpF-like proteins), has been developed; it was optimized by five PCR buffer compounds and the temperature of primer annealing. Detection efficiency of genus-and species-specific primers was determined. The multiplex PCR provides an improved rapid technique for detecting the Yersinia genus and identifying pathogenic species.
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