Development of a multimodal digital micromirror device based microscope for fast label-free imaging and multicolor structured illumination microscopy

Abstract

Fast, long-term superresolution imaging of live cells can reveal biological processes which occur rarely but with fast dynamics. However, these requirements are difficult to realize simultaneously due to the slow speeds of most superresolution techniques and photobleaching and phototoxicity which are ubiquitous in long-term fluorescence imaging. A possible solution is to perform continuous label-free imaging to monitor a sample for behavior of interest, which then triggers high-speed fluorescent superresolution imaging.