Abstract
A new instrument has been developed that is similar in design and concept to the GeMSAEC centrifugal analyzer (Oak Ridge National Laboratory) except that electrochemical detection is used rather than optical detection. The present version has eight channels, each with its own detector. Problems of speed control and sampling synchronization are greatly minimized from those of a multichannel centrifugal analyzer having a single detector system. All eight sample compartments and polarographic cells are contained in a single Teflon rotor, 7.7 cm in diameter. The working electrodes are planar carbon electrodes. The initial application of the analyzer is in kinetic methods involving the rates of enzyme-catalyzed reactions. The initial system chosen was the glucose oxidase system. When the rotor is spun, the solution moves into the sample cell and up against the planar working electrode. The reaction starts and current is measured as a function of time at a constant applied voltage. The current output from each polarographic circuit is connected to a separate channel of a multiplexed analog-to-digital converter. The analyzer is controlled by a dedicated microcomputer system, which sets the polarographic cell voltages, collects the current-time data, and calculates the results.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
