Abstract
To date, methods for quantifying cellular proliferation within the human ovary have relied primarily on static measurements at the time of tissue recovery, such as labeling for ki67 and phosphohistone-H3. Here, we describe a method based on consecutive injection of two thymidine analogues, ethynyl deoxyuridine (EdU) and 5-chloro-2′-deoxyuridine (CldU), in a human ovarian xenograft model, allowing resolution of cellular division within discrete temporal windows of follicle development and observation of a previously undescribed influence of lead follicles on the proliferative activity of surrounding stroma and preantral follicles.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
