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Abstract
A ground sample is homogenised with ethyl acetate, the mixture filtered, and the extract recovered by evaporation of the solvent. The residue is defatted by partition between aqueous methanol and hexane. The aqueous phase is evaporated to dryness and the residue dissolved in dichloromethane and passed through a cartridge of silica. T-2 toxin is eluted with methanol-dichloromethane, the solvents removed and risidue silylated. O-Trimethylsilyl T-2 toxin is detected by gas chromatography-mass spectrometry with monitoring of the ions at m/ z 350 and m/ z 436. The quantitative detection limit is 5 ppb (μ/kg) with an average recovery of 80%.
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