Abstract
Purpose Discomfort is leading factor resulting in discontinued contact lens wear, however the inflammatory mechanisms potentially underpinning discomfort are not well defined. Characterisation of the inflammatory status of epithelial cells and immune cells removed from the ocular surface represents a powerful tool to investigate the coordination of these processes by different cell types. We have developed a mass cytometry method, which enables simultaneous assessment of up to 40 surface or intracellular proteins, to identify the immune cell infiltrate and inflammatory status of epithelial cells in contact lens wearers and validated this using primary corneal epithelial cells.
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