Development of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and Products.

Anna Raysyan,Rudolf J Schneider

doi:10.3390/bios11070231

Abstract

One of the most important chemicals used in the production of polymer plastics and coatings is bisphenol A. However, despite the large number of studies on the toxicity and hormonal activity of BPA, there are still open questions and thus considerable media attention regarding BPA toxicity. Hence, it is necessary to develop a sensitive, simple, cost-efficient, specific, portable, and rapid method for monitoring bisphenol A and for high sample throughput and on-site screening analysis. Lateral flow immunoassays have potential as rapid tests for on-site screening. To meet sensitivity criteria, they must be carefully optimized. A latex microparticle-based LFIA for detection of BPA was developed. The sensitivity of the assay was improved by non-contact printing of spot grids as the control and test lines with careful parameter optimization. Results of the test could be visually evaluated within 10 min with a visual cut-off of 10 µg/L (vLOD). Alternatively, photographs were taken, and image analysis performed to set up a calibration, which allowed for a calculated limit of detection (cLOD) of 0.14 µg/L. The method was validated for thermal paper samples against ELISA and LC–MS/MS as reference methods, showing good agreement with both methods.

Highlights

One of the most important chemicals used in the production of polymer plastics and coatings is bisphenol A
We developed a reproducible Lateral Flow Immunoassay (LFIA) assay, which is cost effective in terms of development and implementation and quantification of the results by readily available software
270–780 μg/L) (Figure S4), which sometimes substitutes bis-phenol A (BPA) in its applications. This is the first work reporting the development of a latex-microparticlebased LFIA technique for the detection of BPA

Summary

Introduction

One of the most important chemicals used in the production of polymer plastics and coatings is bisphenol A. It is necessary to develop a sensitive, simple, cost-efficient, specific, portable, and rapid method for monitoring bisphenol A and for high sample throughput and on-site screening analysis. The rapid increase in the global population has accelerated the production of food, industrial products, and the need for service activities This has, in turn, contributed to the massive growth of the corresponding industrial sectors to meet the high demand for goods of all kind. Another effect is that the number of chemicals used in consumer products is steadily increasing, whereas understanding of human exposure to them and associated human health risks often lag behind. Despite the large number of studies on the toxicity and hormonal activity of BPA [2,3,4], there are still open questions and considerable media attention regarding BPA toxicity

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Discussion

Conclusion