Abstract

In this study, we investigated culture conditions of primary porcine hepatocytes for development of a hybrid artificial liver using polyurethane foam (PUF)/spheroid culture. Porcine hepatocytes in the pores of hydrophilic PUF only formed spheroids spontaneously within 1.5 days of culture, and maintained ammonia detoxification activity in Williams’ E medium supplemented with 10∼20% fetal bovine serum (FBS) for a week. The porcine hepatocyte spheroids in multi-channel (MC) PUF packed-bed module also expressed the highest activities of ammonia detoxification and albumin production at an immobilized cell density of 1.0×107 cells/cm3 module and at an intrachannel medium velocity range from 60 to 80 cm/min.KeywordsPolyurethane FoamAverage Pore SizePerfusion CultureHigh Cell Density CultureSpheroid CultureThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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